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Gene Cluster for Assembly of Pilus Colonization Factor Antigen III of Enterotoxigenic Escherichia coli

机译：产肠毒素大肠杆菌大肠菌群定植因子抗原III装配的基因簇

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The assembly of pilus colonization factor antigen III (CFA/III) of enterotoxigenic Escherichia coli (ETEC) requires the processing of CFA/III major pilin (CofA) by a prepilin peptidase (CofP), similar to other type IV pilus formation systems. CofA is produced initially as a 26.5-kDa preform pilin (prepilin) and then processed to a 20.5-kDa mature pilin by CofP which is predicted to be localized in the inner membrane. In the present experiment, we determined the nucleotide sequence of the whole region for CFA/III formation and identified a cluster of 14 genes, including cofA and cofP. Several proteins encoded by cof genes were similar to previously described proteins, such as the toxin-coregulated pili of Vibrio cholerae and the bundle-forming pili of enteropathogenic E. coli. The G+C content of the cof gene cluster was 37%, which was significantly lower than the average for the E. coli genome (50%). The introduction of a recombinant plasmid containing the cof gene cluster into the E. coli K-12 strain conferred CFA/III biogenesis and the ability of adhesion to the human colon carcinoma cell line Caco-2. This is the first report of a complete nucleotide sequence of the type IV pili found in human ETEC, and our results provide a useful model for studying the molecular mechanism of CFA/III biogenesis and the role of CFA/III in ETEC infection.
机译：肠毒素性大肠杆菌（ETEC）的菌毛定殖因子抗原III（CFA / III）的组装需要通过prepilin肽酶（CofP）处理CFA / III主要菌毛（CofA），类似于其他IV型菌毛形成系统。 CofA最初以26.5 kDa的瓶坯菌毛蛋白（prepilin）的形式生产，然后被CofP加工成20.5 kDa的成熟菌毛蛋白，预计它位于内膜中。在本实验中，我们确定了CFA / III形成的整个区域的核苷酸序列，并鉴定了14个基因的簇，包括cofA和cofP。 cof基因编码的几种蛋白质与先前描述的蛋白质相似，例如霍乱弧菌的毒素结合菌毛和肠致病性大肠杆菌的成束菌毛。 cof基因簇的G + C含量为37％，大大低于大肠杆菌基因组的平均值（50％）。将包含cof基因簇的重组质粒导入大肠杆菌K-12菌株可提高CFA / III的生物发生能力和对人结肠癌细胞系Caco-2的粘附能力。这是人类ETEC中发现的IV型菌毛完整核苷酸序列的首次报道，我们的结果为研究CFA / III生物发生的分子机制以及CFA / III在ETEC感染中的作用提供了有用的模型。

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